Abstract

In order to study the cellular morphology and Myeloid/Erythroid (M/E) ratio in the bone marrow of the adult village weaver birds (Ploceus cucullatus), bone marrow samples were collected from the proximal tibiotarsus bone of 30 clinically-healthy adult village weaver birds during post mortem. The bone marrow smears were fixed and stained using the Romanowsky Giemsa Stain (RGS), then viewed microscopically. Blood samples were also collected ante mortem for routine haematological examination. The results showed that the mean M/E ratio was 0.19, the mean erythroid cell percentage was 72.93, the mean myeloid cell percentage was 19.8 and the mean percentage of all other cells was 0.33. The Myeloid/Erythroid (M/E) ratio obtained from this study is comparable to that of quails. The morphology and distribution of blood cells in the bone marrow of adult village weaver birds were similar with reports from other birds such as ducks and black-head gull. Other findings from this study are comparable to existing data in the literature. The peripheral blood evaluation was within reference ranges. There is need to further determine the mean sizes of all cells of the bone marrow of village weaver birds and compare with that of other birds.

Keywords:

Avian, bone marrow, cell morphology, cytology, myeloid/erythroid ratio, village weaver,

References

1. Almany Magal, R., N. Reznikov, R. Shahar and S. Weiner, 2014. Three-dimensional structure of minipig fibrolamellar bone: Adaptation to axial loading. J. Struct. Biol., 186(2): 253-264.
   CrossRef    PMid:24632449    
2. Bain, B.J., D.M. Clark and B. Wilkins, 2010. Bone Marrow Pathology. 4th Edn., Wiley-Blackwell, Chichester, West Sussex, Hoboken, NJ, pp: 11.
   Direct Link
3. Bounous, D.J. and N.L. Stedman, 2000. Normal Avian Hematology: Chicken and Turkey. In: Feldman, B.F. and J.C. Zink (Eds.), Schalm's Veterinary Hematology. 5th Edn., Philadelphia, Lea & Febiger, USA, pp: 1147-1154.
   
4. Campbell, T.W., 1988. Avian Hematology and Cytology. Iowa State University Press, Iowa, Ames, USA, pp: 3-27.
   
5. Chand, N., S. Singla, K. Sangwan, H. Bansal, D. Bajwa, A. Sharma and S. Goyal, 2015. Diagnosis of hematological and non-hematological disorders using bone marrow aspiration and trephine biopsy (A correlating study). RJPBCS, 7(5): 1259-1268.
   
6. Dean, L.,2005. Blood Groups and Red Cell Antigens [Internet]. Bethesda (MD): National Center for Biotechnology Information (US). Retrieved from: https://www.ncbi.nlm.nih.gov/books/NBK2261/.
   Direct Link
7. Farhi, D.C., 2009. Pathology of Bone Marrow and Blood Cells. 2nd Edn., Lippincott, Williams and Wilkins, Philadelphia, pp: 1-5.
   Direct Link
8. Green, J. P., J. Foerster, and J.N. Lukens, 2003. Wintrobe's Clinical Hematology. 11th Edn., Lippincott Williams and Wilkins Publishers, pp: 25.
   
9. Harvey, J.W., 1984. Canine bone marrow: Normal hematopoiesis, biopsy techniques, and cell identification and evaluation. Comp. Cont. Educ. Pract.,10: 909-925.
   
10. Jain, N.C., 1993. Essentials of Veterinary Hematology. Lea and Febiger, Philadelphia, pp: 8-18.
    Direct Link
11. Kaur, M., A.P. Singh Rana, S. Kapoor and A. Puri, 2014. Diagnostic Value of Bone Marrow Aspiration and Biopsy in Routine Hematology Practice. J. Clin. Diagn. Res., 8(8): FC13-FC16.
    PMid:25302200 PMCid:PMC4190721    
12. Kim, C.H., 2010. Homeostatic and pathogenic extramedullary hematopoiesis. J. Blood Med., 1: 13-19.
    CrossRef    PMid:22282679 PMCid:PMC3262334    
13. Kulikov, E.V., S.B. Seleznev and G.A. Vetoshkina, 2014. Morphological indicators of bone marrow in Guinea fowl of the Volga white breed. Morphology, 145(3): 109-112.
    
14. Kulikov, E., S.B. Seleznev, E.D. Sotnikova, Y.A. Vatnikov, E.V. Kharlitskaya, V.I. Parshina, E.O. Rystsova and N.I. Troshina, 2016. The morphological aspects of bone marrow of guinea fowl of the volga white breed in postembryonic ontogenesis. Res. J. Pharmaceut. Biol. Chem. Sci., 7(5): 1148-1153.
    
15. Lee, S.H., W.N. Erber, A. Porwit, M. Tomonaga and L.C. Peterson, 2008. ICSH guidelines for the standardization of bone marrow specimens and reports. Int. J. Lab. Hematol., 30(5): 349-364.
    CrossRef    PMid:18822060    
16. Lopez, D., L. Lin, J.R. Monaghan, C.R. Cogle, F.J. Bova, M. Maden and E.W. Scott, 2014. Mapping hematopoiesis in a fully regenerative vertebrate: The axolotl. Blood, 124(8): 1232-1241.
    CrossRef    PMid:24802774 PMCid:PMC4141514    
17. Moix, P.A., L. Favre, A. Rosselet and M. Monti, 2008. Bone marrow aspiration and biopsy. Rev. Med. Suisse., 4(177): 2337-2342.
    PMid:19055151    
18. Nazifi, S., M. Tadjalli and M. Mohagheghzadeh, 1999. Normal haematopoiesis cellular components and M/E ratio in the bone marrow of Japanese quail (Coturnix coturnix Japonica). Comp. Haematol. Int., 9(4): 188-192.
    CrossRef    
19. Omonona, A.O., T.A. Adetuga and A.J. Jubril, 2014. Micronucleus as a biomarker of genotoxicity in village weaver bird (Ploceus cucullatus). World. Vet. J., 4(4): 48-53.
    
20. Rubin, R. and D.S. Strayer, 2008. Rubin's Pathology: Clinicopathologic Foundations of Medicine. Wolters Kluwer, Lippincott Williams and Wilkins, Philadelphia, pp: 1368.
    Direct Link
21. Samour, J., 2000. Clinical and diagnostic procedures. In: Avian Medicine. Mosby Publishing, London, pp: 28-42.
    
22. Taichman, R.S., 2005. Blood and bone: Two tissues whose fates are intertwined to create the hematopoietic stem-cell niche. Blood, 105(7): 2631-2639.
    CrossRef    PMid:15585658    
23. Tadjalli, M., S. Nazifi and M. Saeedi Saedi, 1997. Morphological study and determination of M/E ratio of the hematopoietic cells of the duck. Comp. Hematol. Int., 7: 117-121.
    CrossRef    
24. Tadjalli, M., S. Nazifi and M.M. Hadipoor, 2002. Normal haematopoiesis, cellular components and M/E ratio in the bone marrow of the black-headed gull (Larus ridibundus). Comp. Clin. Pathol., 11: 217-222.
    CrossRef    
25. Tadjalli, M., S. Nazifi and M.M. Hadipoor, 2012. Normal haematopoiesis, cellular components and M/E ratio in the bone marrow of the Turkey (Meleagris gallopavo). J. Anim. Sci. Adv., 2(6): 568-575.
    
26. Tadjalli, M., S. Nazifi and R. Haghjoo, 2013. Evaluation of hematopoietic cells and myeloid/erythroid ratio in the bone marrow of the pheasant (Phasianus colchicus). Vet. Res. Forum, 4(2): 119-122.
    PMid:25653783 PMCid:PMC4313013    
27. Tripathy, S. and S. Dudani, 2013. Comparative evaluation of simultaneous bone marrow aspiration and trephine biopsy- Experience from routine hematology practice. Ind. J. Clin. Pract., 24(5): 446-450.
    
28. Weiss, D.J. and K.J. Wardrop, 2010. Schalm's Veterinary Hematology. Wiley-Blackwell, Iowa, Ames, pp: 958-967.http://www.worldcat.org/title/schalms-veterinary-hematology/oclc/1025643907.
    Direct Link

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